Microinjection of dsRNA into mouse one-cell embryos
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1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. steinpau@sas.upenn.edu
Cold Spring Harbor Protocols
|February 12, 2010
Abstract:
RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing in a number of model systems. The following protocol describes delivery of a double-stranded RNA (dsRNA) of choice into mouse one-cell embryos by microinjection. Microinjected embryos can be cultured for up to 4 d, i.e., to the blastocyst stage. The efficiency of knockdown by RNAi can be assayed by quantitative reverse transcriptase (RT)-PCR (qPCR).
