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Extraction of RNA from the plant Kalanchoë daigremontiana
1Department of Plant Biology, University of California, Davis, CA 95616, USA.
Cold Spring Harbor Protocols
|February 12, 2010
Abstract:
This protocol describes how to isolate total RNA from several tissues of Kalanchoë daigremontiana. Total RNA can be isolated by using the TRI-reagent method, scaled up for processing 2 g of tissue, or by using the protocol described here, which gives higher concentrations of high quality RNA. The resulting RNA can be used for various applications including generation of cDNA for reverse transcriptase-PCR (RT-PCR), Northern blots, or other purposes.
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