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Transformation of the moss Physcomitrella patens using T-DNA mutagenesis

David J Cove1, Pierre-François Perroud, Audra J Charron

  • 1Department of Biology, Washington University, St. Louis, MO 63130, USA.

Cold Spring Harbor Protocols
|February 12, 2010
PubMed
Abstract

No abstract available in PubMed .

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Transformation01:26

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...