Devitellinization of living Helobdella (leech) embryos
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David A Weisblat1, Dian-Han Kuo
1Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA. weisblat@berkeley.edu
Cold Spring Harbor Protocols
|February 12, 2010
Abstract:
Embryos of glossiphoniid leeches are enclosed in a thin vitelline envelope until "hatching" (stage 10). This protocol describes the procedure for removing the vitelline envelope from Helobdella robusta and H. sp. (Austin) embryos. This protocol is applicable to embryos of stages 1-9 but is probably more useful for early stages. With careful culturing, the devitellinized embryos can develop normally.
