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Histology Basics and Cell Death Detection in Honeybee Tissue
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Histology Basics and Cell Death Detection in Honeybee Tissue

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Fixation and storage of honeybee (Apis mellifera) tissues

Peter K Dearden1, Elizabeth J Duncan, Megan J Wilson

  • 1Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand. peter.dearden@otago.ac.nz

Cold Spring Harbor Protocols
|February 12, 2010
PubMed
Abstract

No abstract available in PubMed .

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Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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