Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 16, 2026

Murine Left Pulmonary Hilar Clamp Model of Lung Ischemia Reperfusion Injury
06:45

Murine Left Pulmonary Hilar Clamp Model of Lung Ischemia Reperfusion Injury

Published on: April 12, 2024

Lung ischaemia-reperfusion induced gene expression.

Calvin S H Ng1, Connie W C Hui, Song Wan

  • 1Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, China. calvinng@surgery.cuhk.edu.hk

European Journal of Cardio-Thoracic Surgery : Official Journal of the European Association for Cardio-Thoracic Surgery
|February 13, 2010
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond compensatory expansion: Extending 3-dimensional computed tomography volumetry toward lung-preserving local therapy.

The Journal of thoracic and cardiovascular surgery·2026
Same author

Pulsed Electric Field Ablation Reprograms Tumor Immunity and Stimulates Germinal Center Formation in Tertiary Lymphoid Structures in Patients with Non-Small Cell Lung Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Clinical and translational roles of circulating tumor cells in non-small cell and small cell lung cancer: a narrative review.

Journal of thoracic disease·2026
Same author

Intraoperative Assessment of Anatomical Resection Margin Using a Novel Asymmetric Linear Stapler: An Initial Single Institution Experience.

Interdisciplinary cardiovascular and thoracic surgery·2026
Same author

Post-Transbronchial Microwave Ablation Bronchopleural Fistula-A Case Series and Unique Insight.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery·2026
Same author

Integrating Machine Learning and Dynamic Bayesian Networks to Identify the Factors Associated with Subsequent Intrapulmonary Metastasis Classification After Initial Single Primary Lung Cancer.

Cancers·2026

Lung ischaemia-reperfusion injury significantly alters gene expression, impacting apoptosis and inflammation. This study identifies novel genes involved in early lung injury mechanisms, offering insights for therapeutic strategies post-cardiac surgery.

Area of Science:

  • Cardiovascular Surgery
  • Pulmonary Medicine
  • Molecular Biology

Background:

  • Pulmonary dysfunction is a known complication after lung ischaemia-reperfusion (I/R), often seen in post-cardiac surgery patients.
  • This dysfunction is linked to inflammatory responses and cellular apoptosis in the lungs.
  • The early molecular pathways driving this lung injury are not fully understood.

Purpose of the Study:

  • To investigate gene expression changes in the lungs following I/R injury.
  • To identify early molecular mechanisms contributing to lung injury using a rodent model.
  • To explore the role of gene expression in pulmonary apoptosis and cellular processes.

Main Methods:

  • Utilized Sprague-Dawley rodents subjected to varying durations of warm lung ischaemia (60 and 90 minutes) followed by reperfusion.

More Related Videos

A Model of Acute Lung Injury Following Visceral Ischemia-Reperfusion by Supra-Coeliac Aortic Cross Clamping in Rats
06:50

A Model of Acute Lung Injury Following Visceral Ischemia-Reperfusion by Supra-Coeliac Aortic Cross Clamping in Rats

Published on: August 15, 2025

A Mouse Model of Orotracheal Intubation and Ventilated Lung Ischemia Reperfusion Surgery
09:07

A Mouse Model of Orotracheal Intubation and Ventilated Lung Ischemia Reperfusion Surgery

Published on: September 9, 2022

Related Experiment Videos

Last Updated: Jun 16, 2026

Murine Left Pulmonary Hilar Clamp Model of Lung Ischemia Reperfusion Injury
06:45

Murine Left Pulmonary Hilar Clamp Model of Lung Ischemia Reperfusion Injury

Published on: April 12, 2024

A Model of Acute Lung Injury Following Visceral Ischemia-Reperfusion by Supra-Coeliac Aortic Cross Clamping in Rats
06:50

A Model of Acute Lung Injury Following Visceral Ischemia-Reperfusion by Supra-Coeliac Aortic Cross Clamping in Rats

Published on: August 15, 2025

A Mouse Model of Orotracheal Intubation and Ventilated Lung Ischemia Reperfusion Surgery
09:07

A Mouse Model of Orotracheal Intubation and Ventilated Lung Ischemia Reperfusion Surgery

Published on: September 9, 2022

  • Analyzed gene expression changes in lung tissue using rodent DNA microarray chips.
  • Validated key gene expression alterations via reverse transcription polymerase chain reaction (RT-PCR).

    • Main Results:

    • Detected significant expression changes in over 80 genes post-ventilation, with more than 50 genes upregulated over 2-fold.
    • Identified over 50 additional genes with altered expression due to lung I/R, including novel genes.
    • Observed upregulation of genes associated with apoptosis, inflammation, and cell-cycle control.

    Conclusions:

    • Controlled ventilation and early lung I/R induce significant gene expression changes in metabolism, transcription, inflammation, and apoptosis.
    • The study identified novel genes implicated in lung injury, providing new molecular insights.
    • These genetic signatures can inform therapeutic strategies to mitigate pulmonary dysfunction after cardiac surgery.