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Updated: Jun 16, 2026
06:58
Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
Published on: October 7, 2021
RNPomics: defining the ncRNA transcriptome by cDNA library generation from ribonucleo-protein particles
Mathieu Rederstorff1, Stephan H Bernhart, Andrea Tanzer
1Division of Genomics and RNomics, Innsbruck Biocentre, Innsbruck Medical University, Innsbruck and Institute of Theoretical Chemistry, University of Vienna, Vienna, Austria.
Nucleic Acids Research
|February 13, 2010
Summary
Researchers developed a novel RNP method to identify functional non-coding RNAs (ncRNAs). This approach efficiently discovers new small ncRNA candidates involved in ribonucleoprotein particle formation.
Area of Science:
- Molecular Biology
- Genomics
- RNA Biology
Background:
- The human genome transcribes numerous non-coding RNAs (ncRNAs), but identifying functional ones remains challenging.
- Functional ncRNAs in Eukarya are known to form ribonucleoprotein particles (RNPs).
Purpose of the Study:
- To develop and validate an efficient method for identifying functional small ncRNAs.
- To discover novel ncRNA candidates involved in RNP formation.
Main Methods:
- Generation of specialized cDNA libraries from size-fractionated RNPs.
- Validation of ncRNA presence in RNPs using glycerol gradient centrifugation.
- Application of the RNP method to human Hela cells and mouse brain, followed by deep-sequencing and bioinformatical analysis.
Main Results:
- Identification of several hundred ncRNP candidates.
- ncRNA candidates were predominantly found in intergenic and intronic regions, with a notable overrepresentation of intron-derived sequences.
- A subset of ncRNAs mapped to repetitive genomic sequences.
Conclusions:
- The RNP-based approach is an effective strategy for identifying functional small ncRNAs.
- This method facilitates the discovery of novel ncRNAs participating in RNP complexes.
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