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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jun 16, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Photon-Counting H33D Detector for Biological Fluorescence Imaging.

X Michalet¹, O H W Siegmund, J V Vallerga

¹Department of Chemistry & Biochemistry, University of California at Los Angeles, 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.

Nuclear Instruments & Methods in Physics Research. Section A, Accelerators, Spectrometers, Detectors and Associated Equipment

|February 13, 2010

Summary

We created a new photon-counting detector for high-resolution 3D biological imaging. This High-temporal and High-spatial resolution, High-throughput 3-Dimensional (H33D) detector advances fluorescent sample analysis.

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Last Updated: Jun 16, 2026

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Published on: December 10, 2011

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Published on: April 7, 2023

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Published on: August 22, 2019

Area of Science:

Photon-counting detectors
Biological imaging
Advanced detector technology

Background:

Biological imaging requires detectors with high temporal and spatial resolution.
Existing detectors often face limitations in throughput and dimensionality.
Fluorescent samples present unique challenges for high-fidelity imaging.

Purpose of the Study:

To develop and characterize a novel photon-counting detector for 3D biological imaging.
To assess the performance of the detector with various fluorescent samples.
To explore future applications in advanced microscopy and high-throughput screening.

Main Methods:

Development of a detector utilizing an S20 photocathode, microchannel plate stack, and cross delay line anode.
Bench performance characterization of the detector's capabilities.
Preliminary imaging experiments using fluorescent beads, quantum dots, and live cells.

Main Results:

The developed detector achieves high temporal and spatial resolution in 3D.
Successful preliminary imaging of fluorescent beads, quantum dots, and live cells.
Demonstrated potential for photon-counting capabilities in biological imaging.

Conclusions:

The High-temporal and High-spatial resolution, High-throughput 3-Dimensional (H33D) detector is a promising tool for biological imaging.
The detector's performance supports its application in advanced microscopy.
Future iterations hold potential for single-molecule imaging and high-throughput biomolecular interaction studies.