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Updated: Feb 14, 2026

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
Published on: April 25, 2025
Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector
X Michalet1, O H W Siegmund2, J V Vallerga2
1Department of Chemistry & Biochemistry, University of California at Los Angeles 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.
Abstract:
We have recently developed a wide-field photon-counting detector (the H33D detector) having high-temporal and high-spatial resolutions and capable of recording up to 500,000 photons per sec. Its temporal performance has been previously characterized using solutions of fluorescent materials with different lifetimes, and its spatial resolution using sub-diffraction objects (beads and quantum dots). Here we show its application to fluorescence lifetime imaging of live cells and compare its performance to a scanning confocal TCSPC approach. With the expected improvements in photocathode sensitivity and increase in detector throughput, this technology appears as a promising alternative to the current lifetime imaging solutions.
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