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Related Experiment Video

Updated: Jun 16, 2026

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
07:22

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

Two-point fluorophotometer for the human ocular fundus.

C Riva, I Ben-Sira

    Applied Optics
    |February 16, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A novel method enables real-time measurement of fluorescein dilution curves in the human retina. This technique allows for multiple retinal blood flow assessments with minimal dye usage.

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    In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

    Published on: November 11, 2017

    Area of Science:

    • Ophthalmology
    • Medical Imaging
    • Physiology

    Background:

    • Assessing retinal blood flow is crucial for diagnosing and monitoring various eye conditions.
    • Existing methods for measuring retinal circulation may have limitations in precision or require larger dye volumes.

    Purpose of the Study:

    • To develop and describe a new technique for simultaneous, on-line recording of fluorescein dilution curves from two distinct retinal locations.
    • To enable more comprehensive and efficient assessment of retinal hemodynamics.

    Main Methods:

    • A modified Zeiss fundus camera equipped with dual optical fibers was utilized to capture fluorescein dye emissions from two retinal spots.
    • Collected light was transmitted via fiber-optic cables to a photomultiplier for detection.
    • Signals were processed by a two-channel pulse sorter, amplified, and recorded on a two-channel recorder.

    Main Results:

    • The technique successfully generated on-line fluorescein dilution curves from two retinal sites.
    • The system allowed for simultaneous data acquisition from multiple locations.

    Conclusions:

    • This new method provides a feasible approach for dual-site, real-time fluorescein angiography in the human retina.
    • The ability to perform multiple measurements with small fluorescein doses enhances clinical utility and patient safety.