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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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Published on: October 28, 2018

Detectors for single-molecule fluorescence imaging and spectroscopy.

X Michalet1, O H W Siegmund, J V Vallerga

  • 1Department of Chemistry & Biochemistry, University of California at Los Angeles, 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.

Journal of Modern Optics
|February 17, 2010
PubMed
Summary

Single-molecule fluorescence imaging and spectroscopy are powerful tools in biology and physics. Advancements in detector sensitivity and bandwidth are crucial for future single-molecule research.

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Last Updated: Jun 16, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

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Published on: October 28, 2018

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Area of Science:

  • Multidisciplinary research spanning chemistry, biology, and physics.
  • Application of advanced imaging and spectroscopy techniques.

Background:

  • Single-molecule observation techniques offer high sensitivity and specificity, overcoming ensemble averaging limitations.
  • These methods are vital for studying complex biological processes at the molecular level.

Purpose of the Study:

  • To examine the performance requirements for photodetectors in single-molecule experiments.
  • To review the current state-of-the-art in photodetector technology.
  • To discuss future developments in single-photon counting detectors for single-molecule applications.

Main Methods:

  • Review of detector performance metrics including sensitivity and bandwidth.
  • Analysis of existing photodetector technologies for single-molecule applications.
  • Exploration of emerging trends in single-photon counting detectors.

Main Results:

  • Detector sensitivity and bandwidth are critical factors for successful single-molecule experiments.
  • Current photodetector technologies vary in suitability for different single-molecule applications.
  • Ongoing developments aim to enhance detector performance for advanced research.

Conclusions:

  • Optimized photodetector performance is essential for advancing single-molecule imaging and spectroscopy.
  • Future innovations in single-photon counting detectors will further expand research capabilities.
  • These advancements will enable deeper insights into molecular mechanisms and biological processes.