[Antitumor effect of calcium phosphate cement incorporating doxorubicin microspheres]
Hui Zhang1, Zhong-lin Xue, An-min Qi
1Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zhanghuixf@163.com
Objective:
To investigate the antitumor effect of calcium phosphate cement incorporated with doxorubicin microspheres.
Methods:
The absorbance at 490 nm of SaoS-2 cells cultured for 5 days in the media containing the extract of the cement incorporating doxorubicin microspheres was measured using Cell Counting Kit-8. SaoS-2 cells were adjusted to the density of 2x10(7) ml(-1) and injected into the left buttock of nude mouse in the volume of 0.2 ml. The cell suspension (0.1 ml) mixed with an equal volume of the cement extract were injected into the right buttock and on the back of the bilateral ears of nude mice. At 12 days after the cell injection, the tumor tissues were obtained and weighed to calculate the tumor inhibition rate, and the pathological samples were observed with HE staining.
Results:
The extract of the bone cement containing doxorubicin microspheres showed inhibitory effects on the tumor growth in a dose-dependent manner. The tumor inhibition rate reached 61.0% in high-dose group. Tumor necrosis was found in high dose group, but virtually absent in low-dose group.
Conclusions:
CPC containing doxorubicin PLGA microspheres can inhibit tumor cell growth both in vitro and in vivo.
