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Apatite containing aspartic acid for selective protein loading.
M Hafiz Uddin1, T Matsumoto, S Ishihara
1Department of Oromaxillofacial Regeneration, Osaka University, 1-8 Yamada-oka, Suita, 565-0871, Japan.
Hydroxyapatite (HAp) modified with aspartic acid (Asp) shows altered properties, enabling selective basic protein loading. This makes Asp-modified HAp a promising carrier for drug delivery applications, particularly for basic proteins like bFGF and BMP.
Area of Science:
- Biomaterials Science
- Materials Chemistry
- Nanotechnology
Background:
- Hydroxyapatite (HAp) is a key biomaterial for drug delivery and tissue engineering.
- Physico-chemical modifications are crucial for developing advanced HAp carrier materials.
- Acidic amino acids, like aspartic acid (Asp), show affinity for both HAp and basic proteins.
Purpose of the Study:
- To investigate the effects of aspartic acid (Asp) on the physico-chemical properties of hydroxyapatite (HAp).
- To evaluate the potential of Asp-modified HAp as a carrier for basic proteins in drug delivery.
Main Methods:
- Synthesis of HAp in the presence of varying concentrations of Asp.
- Characterization of HAp crystallinity and physico-chemical properties.
- Protein adsorption studies using basic proteins.
Main Results:
- Asp modification modulated HAp crystallinity and physico-chemical properties.
- Asp was trapped within HAp crystals, not incorporated into the lattice.
- Asp-modified HAp exhibited selective loading capacity for basic proteins.
Conclusions:
- Asp-modified HAp demonstrates potential as a bioactive carrier material.
- This material is particularly suitable for the targeted delivery of basic proteins such as bFGF and BMP.
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