Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cystatin C Is Associated with the Extent and Characteristics of Coronary Atherosclerosis in Patients with Preserved Renal Function.

Folia biologica·2017
Same author

Dynamic Change of Focal Fatty Sparing in Non-Alcoholic Fatty Liver Disease after Treatment with Sitagliptin.

Ultrasound international open·2016
Same author

TRPM2 channel deficiency prevents delayed cytosolic Zn2+ accumulation and CA1 pyramidal neuronal death after transient global ischemia.

Cell death & disease·2014
Same author

Limitations of macroscopic fluorescence imaging for the estimation of tumour growth in an orthotopic glioma mouse model.

Folia biologica·2012
Same author

Compartment- and malignance-dependent up-regulation of γ-glutamyltranspeptidase and dipetidylpeptidase-IV activity in human brain gliomas.

Histology and histopathology·2012
Same author

Expression pattern of dipeptidyl peptidase IV activity and/or structure homologues in cancer.

Folia biologica·2009

Related Experiment Video

Updated: Jun 16, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Methods of RNA purification. All ways (should) lead to Rome.

I Vomelová1, Z Vanícková, A Sedo

  • 1Charles University in Prague, First Faculty of Medicine, Institute of Biochemistry and Experimental Oncology, Joint Laboratory of Cancer Cell Biology, and Institute of Physiology AS CR, vvi, Prague, Czech Republic.

Folia Biologica
|February 19, 2010
PubMed
Summary
This summary is machine-generated.

Purifying ribonucleic acid (RNA) is crucial for accurate molecular biology analyses and diagnostics. This review outlines key methods for isolating pure, intact RNA from diverse eukaryotic samples.

More Related Videos

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
08:53

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Published on: May 25, 2019

Related Experiment Videos

Last Updated: Jun 16, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
08:53

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Published on: May 25, 2019

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Ribonucleic acid (RNA) is a vital molecule for numerous laboratory analyses, including diagnostics and basic research.
  • Effective RNA purification is essential for obtaining reliable results in molecular biology techniques.
  • RNA must be purified from various sample types, including tissues, cell lines, and preserved specimens.

Purpose of the Study:

  • To provide an overview of RNA purification methods for eukaryotic, particularly mammalian, tissues.
  • To highlight the importance of pure and intact RNA for downstream applications.
  • To categorize and describe the principal approaches to RNA isolation.

Main Methods:

  • Phenol:chloroform extraction methods.
  • Binding RNA to specific surfaces using chaotropic salts.
  • Isopycnic gradient centrifugation for RNA isolation.

Main Results:

  • Discusses three primary categories of RNA purification techniques.
  • Emphasizes the need for effective and reproducible RNA isolation from heterogeneous sources.
  • Focuses on methods applicable to eukaryotic systems.

Conclusions:

  • RNA purification is a critical prerequisite for accurate molecular diagnostics and research.
  • Understanding different RNA isolation strategies is key to selecting the appropriate method.
  • The review provides a foundational understanding of eukaryotic RNA purification techniques.