Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
Flame Photometry: Lab01:16

Flame Photometry: Lab

In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Flame Photometry: Overview01:02

Flame Photometry: Overview

Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid multiexcitation fluorescence spectroscopy system for in vivo tissue diagnosis.

Applied optics·2010
Same author

Compound parabolic concentrator probe for efficient light collection in spectroscopy of biological tissue.

Applied optics·2010
Same author

Three-dimensional imaging of objects embedded in turbid media with fluorescence and Raman spectroscopy.

Applied optics·2010
Same author

Infrared laser photography with silver-halide emulsion.

Applied optics·2010
Same author

High repetition rate frequency-doubled Nd:YAG laser for airborne bathymetry.

Applied optics·2010
Same author

Efficient cavity dumped dye laser.

Applied optics·2010
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

In Situ Visualization of the Phase Behavior of Oil Samples Under Refinery Process Conditions
11:20

In Situ Visualization of the Phase Behavior of Oil Samples Under Refinery Process Conditions

Published on: February 21, 2017

Absolute oil fluorescence conversion efficiency.

R T Kung, I Itzkan

    Applied Optics
    |February 19, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new method to determine oil conversion efficiencies using fluorescence measurements. It utilizes seawater

    More Related Videos

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
    11:21

    Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

    Published on: June 26, 2010

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    In Situ Visualization of the Phase Behavior of Oil Samples Under Refinery Process Conditions
    11:20

    In Situ Visualization of the Phase Behavior of Oil Samples Under Refinery Process Conditions

    Published on: February 21, 2017

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
    11:21

    Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

    Published on: June 26, 2010

    Area of Science:

    • Remote Sensing
    • Optical Oceanography
    • Spectroscopy

    Background:

    • Accurate determination of oil conversion efficiencies is crucial for environmental monitoring and remediation.
    • Existing methods may require extensive sample preparation or a priori knowledge of oil properties.
    • Fluorescence-based techniques offer a sensitive approach for oil detection and characterization.

    Purpose of the Study:

    • To develop a conceptual method for deducing infinite thickness absolute conversion efficiencies of oils.
    • To establish a reliable method for oil characterization using fluorescence measurements.
    • To assess the impact of signal-to-noise ratio and background fluorescence on measurement accuracy.

    Main Methods:

    • A conceptual method is presented to derive oil conversion efficiencies from finite thickness film fluorescence.
    • The seawater Raman signal is employed as an absolute reference for fluorescence efficiency determination.
    • Temporal behavior of the Raman signal is measured to obtain absolute values.

    Main Results:

    • The method allows for the deduction of absolute conversion efficiencies of oils without prior knowledge.
    • Seawater Raman signal provides a robust reference for accurate fluorescence measurements.
    • The study considers the influence of signal-to-noise and background fluorescence on the results.

    Conclusions:

    • The developed method offers a novel approach for absolute oil conversion efficiency determination.
    • Utilizing the seawater Raman signal enhances the accuracy and reliability of fluorescence-based oil characterization.
    • The findings provide a foundation for improved remote sensing techniques for oil spill assessment.