Related Experiment Video
Updated: Jun 16, 2026

10:16
Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
Published on: March 12, 2019
Summary
This study introduces a new method to intensify photographic images by making silver radioactive. This technique enhances underexposed images and improves detection limits for photographic materials.
Area of Science:
- Radiochemistry
- Photographic Science
- Analytical Chemistry
Background:
- Photographic emulsions capture images using silver halide crystals.
- Image intensification is crucial for enhancing faint signals and improving detection limits.
- Existing methods for intensifying photographic images have limitations in practicality and effectiveness.
Purpose of the Study:
- To develop a novel and practical chemical method for intensifying photographic images.
- To enhance the density and contrast of images on developed photographic emulsions.
- To increase the sensitivity and detection limits of photographic detection.
Main Methods:
- Making the image silver radioactive by treating developed photographic emulsions with thiourea labeled with sulfur-35 (35S).
- Exposing a second, unexposed photographic emulsion to the radiation emitted from the activated silver in the first emulsion.
- Analyzing the resulting autoradiograph for increases in density and contrast.
Main Results:
- Achieved significant increases in image density and contrast through autoradiography.
- Demonstrated speed increases of 13x and contrast increases of 18x with Kodak Plus-X film.
- Validated the practical application of the radioactive silver intensification method in a laboratory setting.
Conclusions:
- The described chemical activation method using sulfur-35 labeled thiourea offers a practical approach for photographic image intensification.
- This technique effectively enhances underexposed photographic emulsions, expanding the limits of photographic detection.
- The method provides substantial improvements in image density and contrast, making it valuable for scientific and technical imaging.

