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Updated: Jun 16, 2026
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
Published on: December 9, 2022
A biomarker panel to discriminate between systemic inflammatory response syndrome and sepsis and sepsis severity
Chamindie Punyadeera1, E Marion Schneider, Dave Schaffer
1Department of Molecular Diagnostics, Philips Research, High Tech Campus 12a, 5656 AE Eindhoven, The Netherlands, Germany.
Introduction:
In this study, we report on initial efforts to discover putative biomarkers for differential diagnosis of a systemic inflammatory response syndrome (SIRS) versus sepsis; and different stages of sepsis. In addition, we also investigated whether there are proteins that can discriminate between patients who survived sepsis from those who did not.
Materials And Methods:
Our study group consisted of 16 patients, of which 6 died and 10 survived. We daily measured 28 plasma proteins, for the whole stay of the patients in the ICU.
Results:
We observed that metalloproteinases and sE-selectin play a role in the distinction between SIRS and sepsis, and that IL-1alpha, IP-10, sTNF-R2 and sFas appear to be indicative for the progression from sepsis to septic shock. A combined measurement of MMP-3, -10, IL-1alpha, IP-10, sIL-2R, sFas, sTNF-R1, sRAGE, GM-CSF, IL-1beta and Eotaxin allows for a good separation of patients that survived from those that died (mortality prediction with a sensitivity of 79% and specificity of 86%). Correlation analysis suggests a novel interaction between IL-1alpha and IP-10.
Conclusion:
The marker panel is ready to be verified in a validation study with or without therapeutic intervention.
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