Ultra-fast HPLC-ICP-MS analysis of oxaliplatin in patient urine
Gunda Koellensperger1, Stephan Hann2
1Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Applied Life Sciences, BOKU, Vienna, Muthgasse 18, 1190, Vienna, Austria.
Abstract:
A novel method for rapid HPLC-ICP-MS analysis of oxaliplatin in human urine was developed implementing a stationary HPLC phase with a particle size of 1.8 microm. The method allowed a cycle time of <1 min at a HPLC flow rate of 0.9 mL min(-1). Procedural limits of detection of 0.05 microg L(-1) oxaliplatin (150 fg on column) were obtained. Analysis of oxaliplatin in patient urine showed that accurate quantification of the intact drug demanded for storage at -80 degrees C and rapid measurement after thawing.
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