Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak Histories.

Microorganisms·2021

Same author

Evaluation of the MC-Media Pad® Rapid Aerobic Count Device for the Enumeration of Total Aerobic Counts in a Variety of Foods: Collaborative Study, First Action Official MethodSM 2019.02.

Journal of AOAC International·2020

Same author

Evaluation of the MC-Media Pad<sup>TM</sup> Yeast and Mold Device for the Enumeration of Yeast and Mold: Collaborative Study, First Action 2018.02.

Journal of AOAC International·2019

Same author

Evidence of a reverse side-chain effect of tris(pentafluoroethyl)trifluorophosphate [FAP]-based ionic liquids against pathogenic bacteria.

Ecotoxicology and environmental safety·2017

Same author

Predictability of ionic liquid toxicity from a SAR study on different systematic levels of pathogenic bacteria.

Ecotoxicology and environmental safety·2017

Same author

Rapid testing and quantification of Salmonella in ileocaecal lymph nodes of Austrian pigs slaughtered for consumption.

Research in veterinary science·2014

Same journal

Single-Laboratory Validation of Simultaneous Determination of Aflatoxins in Nutraceuticals following immune-affinity Column Cleanup and Liquid Chromatography Tandem Mass Spectrometry Analysis.

Journal of AOAC International·2026

Same journal

Determination of Bromoform in Seaweed, Oil, and Animal Feed by GC-MS/MS: AOAC Official Method 2026.01, First Action.

Journal of AOAC International·2026

Same journal

ICRF-Assisted Box-Behnken Design and Optimization for Rapid UPLC-PDA Determination of Dorzolamide Hydrochloride and Timolol Maleate in an Ophthalmic Preparation.

Journal of AOAC International·2026

Same journal

A Green UPLC-UV Method for L-Ascorbic Acid Determination Based on a Biodegradable Chelating Agent and Synergistic Hydrophobic-Electrostatic Interactions.

Journal of AOAC International·2026

Same journal

Quantification of 24 Inorganic Impurities in Vismodegib and Idelalisib Anti-Cancer Drug Substances by Using ICP-MS.

Journal of AOAC International·2026

Same journal

Comprehensive Comparison of ISO-GUM and Monte Carlo Simulation for Uncertainty Assessment in HPLC Statin Quantification with single and multi-point calibrations.

Journal of AOAC International·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

Singlepath Salmonella. Performance Tested Method 060401.

Charlotte Lindhardt¹, Holger Schönenbrücher, Jörg Slaghuis

¹Merck KGaA, Frankfurter Strasse 250, 64293 Darmstadt, Germany. charlotte.lindhardt@merck.de

Journal of AOAC International

|February 20, 2010

Summary

This summary is machine-generated.

The Singlepath Salmonella assay effectively detects Salmonella in creamy peanut butter, showing no significant difference compared to the FDA-BAM reference method, even at low contamination levels. This validates its use for presumptive Salmonella detection in diverse food matrices.

More Related Videos

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

Related Experiment Videos

Last Updated: Jun 16, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

Area of Science:

Food Microbiology
Diagnostic Assay Development
Food Safety Testing

Background:

Salmonella spp. are significant foodborne pathogens requiring reliable detection methods.
The Singlepath Salmonella assay, a lateral flow test, was previously validated for various food types.
Emergency validation is crucial for assessing methods in novel or challenging food matrices.

Purpose of the Study:

To validate the Singlepath Salmonella assay for detecting Salmonella enterica serovar Typhimurium in creamy peanut butter.
To compare the performance of the Singlepath Salmonella assay against the FDA-BAM reference method in a challenging food matrix.

Main Methods:

Creamy peanut butter samples were inoculated with Salmonella enterica serovar Typhimurium at varying concentrations.
Samples were tested using the Singlepath Salmonella immunochromatographic assay.
Results were compared statistically (Chi-square test) to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) reference method.

Main Results:

At low contamination levels (1.08 CFU/25 g), the Chi-square value of 0.5 indicated no significant difference between Singlepath Salmonella and the FDA-BAM method.
100% agreement was observed between the Singlepath Salmonella assay and the FDA-BAM method for high-level contamination and uninoculated control samples.
The assay demonstrated effective presumptive detection of Salmonella in creamy peanut butter.

Conclusions:

The Singlepath Salmonella assay is a reliable method for the presumptive qualitative detection of Salmonella spp. in creamy peanut butter.
The assay's performance is comparable to the established FDA-BAM reference method, supporting its utility in food safety testing.
This validation extends the applicability of the Singlepath Salmonella assay to a wider range of food products.