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Updated: Jun 16, 2026
Gold Nanoparticle Synthesis
Published on: July 10, 2021
Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates
Dragoslav Zikich1, Natalia Borovok, Tatiana Molotsky
1Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, The Center for Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, 69978.
Abstract:
In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiol-functionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo(-) polymerase in the presence of dCTP and dGTP.
