Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Complex Ions03:45

Formation of Complex Ions

A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...
Stereoisomerism02:52

Stereoisomerism

Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
Acid Halides to Ketones: Gilman Reagent01:14

Acid Halides to Ketones: Gilman Reagent

Lithium dialkyl cuprate, also known as Gilman reagents, selectively reduces acid halides to ketones. The acid chloride is treated with Gilman reagent at −78 °C in the presence of ether solution to produce a ketone in good yield.
As shown below, the mechanism proceeds in two steps. First, one of the alkyl groups of the reagent acts as a nucleophile and attacks the acyl carbon of the acid chloride to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen double...
Aldehydes and Ketones with HCN: Cyanohydrin Formation Overview01:32

Aldehydes and Ketones with HCN: Cyanohydrin Formation Overview

Cyanohydrins are compounds that contain –CN and –OH groups on the same carbon atom. They are formed by the nucleophilic addition of the cyanide ions to the carbonyl group. Cyanide ions are highly basic and nucleophilic and can be generated from HCN under aqueous conditions. However, since HCN is a weak acid, the number of cyanide ions generated is very small. Hence, a small amount of base or KCN/NaCN is added to HCN to increase the concentration of the cyanide ions in the reaction mixture.
Complexation Equilibria: The Chelate Effect01:19

Complexation Equilibria: The Chelate Effect

In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolotheranostics of pancreatic cancer by targeting choline, glutamine, and glucose transporters with photoimmunotherapy.

Npj imaging·2026
Same author

Selective Hydrolysis of Heterooligosaccharides by Poly(acrylate) Gel Catalysts.

ACS catalysis·2024
Same author

Developing Catalysts for the Hydrolysis of Glycosidic Bonds in Oligosaccharides Using a Spectrophotometric Screening Assay.

ACS catalysis·2024
Same author

Photoimmunotheranostics of epithelioid sarcoma by targeting CD44 or EGFR.

Translational oncology·2024
Same author

Functional roles of FAP-α in metabolism, migration and invasion of human cancer cells.

Frontiers in oncology·2023
Same author

Evaluating near-infrared photoimmunotherapy for targeting fibroblast activation protein-α expressing cells in vitro and in vivo.

Cancer science·2022
Same journal

Solvent Coordination-Induced Synergistic Phase, Facet, and Defect Engineering of CdS for Photocatalytic Hydrogen Evolution.

Inorganic chemistry·2026
Same journal

Tailoring the Electron-Enriched Microenvironment of UiO-66 via Thiol Functionalization to Boost Non-Thermal Plasma CO<sub>2</sub> Conversion.

Inorganic chemistry·2026
Same journal

Nonporous Self-Assembled Pd(II) Coordination Cage Enabling Dual Capture of Iodine and Methyl Iodide.

Inorganic chemistry·2026
Same journal

A Three-Dimensional Organic-Inorganic Hybrid Perovskite-Type Molecular Ferroelectric Material [3.2.2-H<sub>2</sub>dabcn]Rb(NO<sub>3</sub>)<sub>3</sub>.

Inorganic chemistry·2026
Same journal

Nonlinear Optical-Active NaAlP<sub>2</sub>S<sub>6</sub> Synthesized by the MOBQ Method: Synthesis, Structure, and Optical Properties.

Inorganic chemistry·2026
Same journal

Ligand-Controlled Redox and Photophysical Properties in Photoluminescent Tris-Heteroleptic Ru(II) Pyridyl-Phosphonium Ylide Complexes.

Inorganic chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
06:31

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

Published on: March 19, 2020

Evaluating binuclear copper(II) complexes for glycoside hydrolysis.

Susanne Striegler1, Natasha A Dunaway, Moses G Gichinga

  • 1Department of Chemistry and Biochemistry, 179 Chemistry Building, Auburn University, Auburn, Alabama 36849, USA. susanne.striegler@auburn.edu

Inorganic Chemistry
|February 20, 2010
PubMed
Summary
This summary is machine-generated.

Three binuclear copper(II) complexes show significant glycosidase-like activity, accelerating the hydrolysis of nitrophenylglycopyranosides. These complexes demonstrate potential as catalysts in glycosidic bond cleavage reactions.

More Related Videos

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota
13:35

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Published on: May 23, 2025

Related Experiment Videos

Last Updated: Jun 16, 2026

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
06:31

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

Published on: March 19, 2020

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota
13:35

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Published on: May 23, 2025

Area of Science:

  • Coordination Chemistry
  • Biomimetic Catalysis
  • Enzyme Mimics

Background:

  • Glycosidases are crucial enzymes involved in carbohydrate metabolism.
  • Developing synthetic catalysts that mimic glycosidase activity is an active area of research.
  • Copper complexes are known to exhibit diverse catalytic properties.

Purpose of the Study:

  • To synthesize and characterize binuclear copper(II) complexes.
  • To investigate the glycosidase-like activity of these complexes.
  • To understand the relationship between complex structure and catalytic efficiency.

Main Methods:

  • X-ray diffraction for solid-state structural characterization.
  • UV/vis spectrophotometric titration for solution behavior analysis.
  • Enzyme kinetic assays using nitrophenylglycopyranosides as substrates.

Main Results:

  • Three binuclear copper(II) complexes were successfully synthesized and characterized.
  • Structural analysis revealed similar Cu...Cu distances (approx. 3.5 Å) in two complexes.
  • Spectrophotometric titrations indicated significant differences in solution composition.
  • Catalytic activity demonstrated up to 11,000-fold acceleration of nitrophenylglycopyranoside hydrolysis at pH 10.5.

Conclusions:

  • The binuclear copper(II) complexes exhibit potent glycosidase-like activity.
  • Catalytic efficiency is influenced by the complex's solution behavior.
  • These complexes represent promising biomimetic catalysts for glycosidic bond hydrolysis.