Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 16, 2026

Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
11:44

Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities

Published on: October 2, 2018

Synthetic study on cystinyl peptides using solution and solid phase methodology: human IgG1 hinge region.

P Niederhafner1, V Gut, J Jezek

  • 1Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovo nám. 2, 166 10, Prague 6, Czech Republic.

Amino Acids
|February 20, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strangulation ileus caused by malposition of the ureter.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2024
Same author

Measurement of Lepton-Jet Correlation in Deep-Inelastic Scattering with the H1 Detector Using Machine Learning for Unfolding.

Physical review letters·2022
Same author

[Risk Factors for the Development of Distal Tibiofibular Synostosis after Unstable Ankle Fractures].

Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca·2022
Same author

[Comparison of Clinical Outcomes of Displaced Diaphyseal Clavicle Fractures].

Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca·2022
Same author

S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes.

Biochimica et biophysica acta. Molecular basis of disease·2020
Same author

Determination of the strong coupling constant <math> </math> in next-to-next-to-leading order QCD using H1 jet cross section measurements: H1 Collaboration.

The European physical journal. C, Particles and fields·2020

Researchers developed efficient synthetic methods for cystinyl peptides, crucial components of immunoglobulin G1. Pure oxygen accelerated disulfide bond formation, reducing impurities and improving synthesis speed for these important biomolecules.

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Immunology

Background:

  • Cystinyl peptides are essential structural components of immunoglobulin G1 (IgG1).
  • Efficient synthesis of these peptides is crucial for biochemical and immunological research.
  • Current methods for synthesizing cystinyl peptides can be time-consuming and prone to side reactions.

Purpose of the Study:

  • To develop and optimize synthetic methodologies for cystinyl peptides, focusing on the central hinge region of IgG1.
  • To improve the efficiency and purity of synthetic cystinyl peptides using both solution and solid-phase techniques.
  • To explore novel strategies for disulfide bond formation and peptide coupling.

Main Methods:

  • Solid-phase peptide synthesis (SPPS) of a hexadecapeptide (1c) from the IgG1 hinge region.

More Related Videos

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Related Experiment Videos

Last Updated: Jun 16, 2026

Synthesis and Structure Determination of &#181;-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
11:44

Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities

Published on: October 2, 2018

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

  • Solution-phase synthesis of shortened bis-cystinyl analogs (2k and 3d) using classical and polyethylene glycol (PEG6000) methods.
  • Oxidation of linear precursors using pure oxygen versus air to form disulfide bonds.
  • Utilizing a disulfide synthon (Boc-Cys-OPfp)2 for parallel peptide chain alignment.
  • Employing lysine linkers on PEG6000 to enhance loading capacity.
  • Introducing a leucine residue for specific enzymatic cleavage by thermolysin.

    • Main Results:

    • Pure oxygen significantly accelerated disulfide bond formation (fourfold increase) in solid-phase synthesis, yielding a purer hexadecapeptide (1c).
    • Both classical and PEG6000 solution methods successfully produced bis-cystinyl analogs (2k and 3d) with natural parallel alignment.
    • The PEG6000 strategy, with lysine linkers, demonstrated enhanced loading capacity.
    • Enzymatic cleavage from PEG6000 was facilitated by the carboxy-terminal leucine residue.

    Conclusions:

    • Optimized synthetic routes, particularly using pure oxygen, enhance the efficiency and purity of cystinyl peptide synthesis.
    • Polyethylene glycol-based strategies offer advantages in loading capacity and controlled cleavage for peptide synthesis.
    • These improved methods provide valuable tools for producing well-defined cystinyl peptides for further research.