Side population increase after simulated transient ischemia in human dental pulp cell
Jinming Wang1, Xi Wei, Junqi Ling
1Department of Operative Dentistry and Endodontics, Sun Yat-sen University, Guangdong, China.
Journal of Endodontics
|February 23, 2010
Summary
Simulated ischemia increases side population (SP) stem cells in dental pulp. These SP cells may play a crucial role in the repair and regeneration of dental pulp tissue after injury.
Area of Science:
- Dental Stem Cells
- Tissue Regeneration
- Ischemic Conditions
Background:
- Dental pulp is susceptible to ischemia due to limited blood supply.
- Resident stem cells are crucial for post-ischemic regeneration.
- Understanding stem cell behavior under ischemia is vital for pulp repair.
Purpose of the Study:
- Investigate the impact of simulated ischemia on human dental pulp side population (SP) stem cells.
- Elucidate the role of SP stem cells in post-ischemic dental pulp repair and regeneration.
Main Methods:
- Simulated ischemia induced via serum deprivation and hypoxia (2% O2, 2% serum).
- Assessed dental pulp cell (DPC) proliferation using MTT assay.
- Quantified SP fraction via Hoechst 33342 flow cytometry and ABCG2 expression via immunofluorescence.
- Measured ABCG2 and OCT4 mRNA levels using real-time PCR.
Main Results:
- DPC proliferation decreased under ischemic conditions.
- SP proportion and ABCG2 expression significantly increased after 24 and 48 hours of simulated ischemia.
- ABCG2 and OCT4 mRNA levels more than tripled in the 48-hour ischemic group.
Conclusions:
- Transient simulated ischemia significantly elevates the side population in dental pulp cells.
- Increased SP cells suggest their active participation in the post-ischemic repair and regeneration of dental pulp.
