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Related Experiment Videos

Is 20S RNA naked?

W R Widner1, Y Matsumoto, R B Wickner

  • 1Section on Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Molecular and Cellular Biology
|May 1, 1991
PubMed
Summary

The 20S RNA virus in yeast is not associated with heat shock protein Hsp26. This protein, previously thought to bind the viral RNA, plays no clear role in the virus's life cycle.

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Area of Science:

  • Virology
  • Molecular Biology
  • Yeast Genetics

Background:

  • Saccharomyces cerevisiae harbors the 20S RNA, a single-stranded, circular RNA virus.
  • Previous research indicated the 20S RNA associates with a 23-kilodalton protein in a 32S ribonucleoprotein particle.

Purpose of the Study:

  • To identify the protein associated with the 20S RNA of Saccharomyces cerevisiae.
  • To clarify the role of this protein in the viral life cycle.

Main Methods:

  • Protein identification techniques.
  • Analysis of ribonucleoprotein particle composition.
  • Investigation of protein-virus interactions.

Main Results:

  • The 23-kilodalton protein associated with the 32S particle is identified as heat shock protein Hsp26.
  • Hsp26 is encoded by the yeast chromosome.
  • Evidence suggests Hsp26 is not associated with the 20S RNA.
  • Hsp26 does not appear to play a role in the 20S RNA virus life cycle.

Conclusions:

  • Heat shock protein Hsp26 is not a component of the 20S RNA viral particle in Saccharomyces cerevisiae.
  • The previously proposed association between Hsp26 and 20S RNA is incorrect.
  • The function of Hsp26 in relation to this specific viral RNA remains undetermined.

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