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Related Experiment Video

Updated: Jun 16, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Gold nanoparticles based sandwich electrochemical immunosensor.

Gautham Kumar Ahirwal1, Chanchal K Mitra

  • 1Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Andhra Pradesh, 500046, India.

Biosensors & Bioelectronics
|February 23, 2010
PubMed
Summary

This study developed a gold nanoparticle-based biosensor for detecting analytes using a sandwich electrochemical immunoassay. The novel biosensor achieved a low detection limit, demonstrating its potential for clinical diagnostics.

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Area of Science:

  • Nanotechnology
  • Electrochemistry
  • Immunochemistry

Background:

  • Gold nanoparticles (AuNPs) offer unique electrochemical properties.
  • Antibody immobilization is crucial for biosensor development.
  • Electrochemical immunoassays provide sensitive detection methods.

Purpose of the Study:

  • To develop a novel electrochemical immunoassay using AuNPs.
  • To covalently attach antibodies to AuNPs for enhanced stability and orientation.
  • To detect analytes with high sensitivity and specificity.

Main Methods:

  • Fabrication of a gold electrode modified with AuNPs and antibodies (Ab(1)).
  • Sandwich electrochemical immunoassay utilizing secondary antibodies labeled with Horse Radish Peroxidase (HRP).

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Last Updated: Jun 16, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

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Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

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  • Detection via cyclic voltammetry and electrochemical impedance spectroscopy with TMB as the electroactive dye.

    • Main Results:

    • Cyclic voltammetry revealed distinct peaks related to TMB oxidation/reduction and antibody-antigen complex formation.
    • The biosensor demonstrated a low detection limit of 2 ng/mL.
    • Electrochemical impedance spectroscopy confirmed successful antigen-antibody complex formation and electrode assembly.

    Conclusions:

    • AuNPs efficiently preserve antibody activity and orientation for biosensor applications.
    • The developed AuNP-based electrochemical immunoassay is a promising platform for clinical diagnostics.
    • This method offers a sensitive and reliable approach for analyte detection.