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Related Concept Videos

Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview

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Related Experiment Video

Updated: Jun 16, 2026

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
15:01

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

A novel link to base excision repair?

David M Wilson1, Michael M Seidman

  • 1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. wilsonda@mail.nih.gov

Trends in Biochemical Sciences
|February 23, 2010
PubMed
Summary
This summary is machine-generated.

DNA interstrand crosslinks (ICLs), which block DNA replication and cause cell death, are processed by DNA repair pathways. Emerging evidence links the base excision repair pathway to ICL resolution, alongside nucleotide excision repair and homologous recombination.

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Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
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Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

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Last Updated: Jun 16, 2026

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15:01

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

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Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA interstrand crosslinks (ICLs) are DNA lesions that covalently bind both DNA strands.
  • ICLs block essential cellular processes like DNA replication and transcription, leading to cell death.
  • ICLs can be induced by endogenous compounds (e.g., malondialdehyde) or exogenous agents (e.g., chemotherapy drugs like platinum compounds and alkylators).

Purpose of the Study:

  • To investigate the role of DNA repair pathways in resolving DNA interstrand crosslinks (ICLs).
  • To explore the potential involvement of the base excision repair (BER) pathway in processing ICLs.

Main Methods:

  • Review of existing literature on DNA repair mechanisms.
  • Analysis of recent research findings and historical reports on ICL processing.

Main Results:

  • Nucleotide excision repair (NER) and homologous recombination (HR) are established pathways for ICL resolution.
  • Recent studies, supported by earlier findings, indicate a potential role for the base excision repair (BER) pathway in ICL processing.

Conclusions:

  • The understanding of ICL repair is expanding beyond NER and HR.
  • The base excision repair pathway may represent a novel mechanism for resolving DNA interstrand crosslinks, complementing existing pathways.