Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

9.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.8K
Drug Discovery: Overview01:26

Drug Discovery: Overview

11.8K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
11.8K
Nursing Clinical Information System01:27

Nursing Clinical Information System

1.3K
Nursing Clinical Information System (NCIS)
A Nursing Clinical Information System (NCIS) is a specialized type of healthcare information system tailored to meet the unique needs of nursing practice. It incorporates the principles of nursing informatics to streamline information management and improve the quality of care delivery.
Critical attributes of NCIS include:
1.3K
Clinical Trials01:16

Clinical Trials

10.8K
Clinical trials are prospective experimental studies conducted on humans to determine the safety and efficacy of treatments, drugs, diet methods, and medical devices. Using statistics in clinical trials enables researchers to derive reasonable and accurate conclusions from the collected data, allowing them to make wise decisions in uncertain situations. In medical research, statistical methods are crucial for preventing errors and bias.
There are four phases in a clinical trial. A phase one...
10.8K
Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

899
Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
899
Clinical Trials: Overview01:11

Clinical Trials: Overview

5.0K
Clinical development focuses on how the drug will interact with the human body and encompasses four key phases of clinical trials, each serving a specific purpose in assessing the safety and effectiveness of new drugs. These phases overlap and build upon one another. Phase I involves a small group of healthy volunteers (typically 20-80 individuals) or, in cases where significant toxicity is expected, patients with the targeted disease, such as cancer or AIDS. The volunteers are tested for...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Role of LVV-H7 in Alcohol-Induced Reward Mechanisms.

Addiction biology·2025
Same author

Seasonal Proteome Variations in <i>Orbicella faveolata</i> Reveal Molecular Thermal Stress Adaptations.

Proteomes·2024
Same author

Oligopeptide-based molecular labelling of (bio)degradable polyester biomaterials.

International journal of biological macromolecules·2024
Same author

Change of histone H3 lysine 14 acetylation stoichiometry in human monocyte derived macrophages as determined by MS-based absolute targeted quantitative proteomic approach: HIV infection and methamphetamine exposure.

Clinical proteomics·2023
Same author

Corrigendum to "Discovery of candidate HIV-1 latency biomarkers using an OMICs approach" [Virology (2021 Jun) 558 86-95, Epub 2021 Mar 11].

Virology·2023
Same author

HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages.

Journal of neurovirology·2022

Related Experiment Video

Updated: Feb 7, 2026

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

1.1K

Biomarker discovery and clinical proteomics.

Jerzy Silberring1, Pawel Ciborowski

  • 1Department of Biochemistry and Neurobiology, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland.

Trends in Analytical Chemistry : TRAC
|February 23, 2010
PubMed
Summary

Developing new diagnostic biomarkers for diseases is challenging due to variability in proteomics studies. Advancements in protein arrays could improve biomarker discovery and validation for drug development.

More Related Videos

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

17.2K
Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

14.0K

Related Experiment Videos

Last Updated: Feb 7, 2026

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

1.1K
Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

17.2K
Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

14.0K

Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Translational Medicine

Background:

  • Urgent need for novel biomarkers to accelerate drug development and disease treatment.
  • Proteomics studies frequently face variability in patient cohort assembly, sample preparation, and data analysis, hindering biomarker discovery.
  • Current validation methods often lack desired sensitivity, specificity, and reproducibility.

Purpose of the Study:

  • To highlight the challenges in biomarker discovery within proteomics.
  • To discuss the limitations of existing proteomics methods for clinical sample screening.
  • To propose protein arrays as a potential solution for high-throughput biomarker discovery.

Main Methods:

  • Review of current challenges in clinical and translational proteomics.
  • Analysis of variability in sample preparation, profiling methods, and data analysis protocols.
  • Discussion on the throughput limitations of mass spectrometry and gel-based methods.

Main Results:

  • Biomarker discovery lags due to inherent variability in proteomics workflows.
  • Existing validation steps frequently fail to achieve adequate sensitivity, specificity, or reproducibility.
  • Mass spectrometry and gel-based techniques lack the throughput for large-scale clinical sample screening.

Conclusions:

  • Despite obstacles, proteomics provides valuable insights into disease mechanisms.
  • Further development of protein arrays is crucial for overcoming throughput limitations in biomarker discovery.
  • Improved methods are essential for reliable and reproducible biomarker identification in clinical proteomics.