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Grb10 interacts with Bim L and inhibits apoptosis
Zhi-qian Hu1, Jia-yi Zhang, Chao-neng Ji
1State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, 200433, People's Republic of China.
Molecular Biology Reports
|February 23, 2010
Summary
Growth factor receptor-bound protein 10 (Grb10) inhibits Bim-induced apoptosis by binding to Bim
Area of Science:
- Cell biology
- Molecular biology
- Apoptosis regulation
Background:
- Bim is a key regulator of the intrinsic apoptotic pathway.
- The precise mechanisms controlling Bim's proapoptotic activity require further elucidation.
Purpose of the Study:
- To identify proteins interacting with Bim.
- To investigate the functional consequences of Bim-Grb10 interaction on apoptosis.
Main Methods:
- Yeast two-hybrid screening of a human fetal cDNA library.
- Co-immunoprecipitation and intracellular co-localization assays.
- Yeast mating assays to map interaction domains.
Main Results:
- Growth factor receptor-bound protein 10 (Grb10) was identified as a Bim-interacting protein.
- Grb10 binds to the dynein binding domain (DBD) of Bim.
- Grb10 inhibits Bim-induced apoptosis, a function dependent on phosphorylation sites within the Bim DBD.
Conclusions:
- Grb10 directly interacts with Bim L.
- Grb10 negatively regulates Bim's proapoptotic function in a phosphorylation-dependent manner.
- This interaction offers a novel regulatory mechanism for apoptosis.
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