Related Experiment Video
Updated: Jun 16, 2026

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
Published on: April 13, 2018
The dependence of enhanced lysosomal activity on the cellular aging of bovine aortic endothelial cells
Jihee Yoon1, Ki-Ju Kim, Young-Woo Choi
1Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
Abstract:
Lysosomes are a cell organelle type processing antimicrobial activity. Here, we investigate the lysosomal activity in a primary cell line, bovine aortic endothelial cells (BAECs), during cellular aging, based on the antimicrobial activity of lysosomes isolated from BAECs at cell passages 4, 6, 8, and 10. Cytochemical analysis of lysosomes with LysoTracker reagent revealed the number of lysosome-like organelles surrounding the nucleus initially increased drastically in the BAECs and continued increasing gradually until passage 10. The lysosomes isolated from each successive passage of BAECs exhibited increased antimicrobial activity against Escherichia coli, and, in addition, an age-dependent increase in lysosome intensity coincided with increased lysosomal antimicrobial activity.
Related Concept Videos
The Effect of Aging on Tissues
Lysosomal Hydrolases
Maturation of Endosomes
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...

