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Related Experiment Video

Updated: Jun 16, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

A microfluidic oligonucleotide synthesizer.

Cheng-Chung Lee¹, Thomas M Snyder, Stephen R Quake

¹Department of Bioengineering, California Institute of Technology, Pasadena, CA 91125, USA.

Nucleic Acids Research

|February 24, 2010

Summary

This summary is machine-generated.

De novo gene synthesis is now more affordable thanks to a new microfluidic platform. This technology reduces reagent costs by 100-fold, enabling faster and cheaper DNA construct generation for research.

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High-throughput Protein Expression Generator Using a Microfluidic Platform

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Published on: August 23, 2012

Area of Science:

Synthetic Biology
Molecular Biology
Biotechnology

Background:

De novo gene and genome synthesis allows for the creation of custom DNA sequences without relying on existing templates.
High costs currently limit the widespread adoption and application of de novo DNA constructs in biological research.
Traditional genetic engineering methods require pre-existing templates, which de novo synthesis bypasses.

Purpose of the Study:

To develop a cost-effective method for de novo DNA synthesis.
To create a microfluidic platform for parallel oligonucleotide synthesis.
To enable the generation of synthetic DNA constructs without the need for amplification.

Main Methods:

A microfluidic platform was designed for parallel solid-phase synthesis of oligonucleotides.
Reagent consumption was reduced by approximately 100-fold compared to conventional methods.
Solid-phase synthesis was performed at a scale of approximately 100 pmol per oligonucleotide.

Main Results:

The microfluidic platform successfully synthesized sixteen oligonucleotides in parallel.
The synthesized oligonucleotides were used in a ligation-mediated assembly.
DNA constructs of approximately 200 bp were generated, suitable for direct use without amplification.

Conclusions:

The developed microfluidic platform significantly reduces the cost of de novo gene synthesis.
This technology facilitates the production of synthetic DNA constructs at a scale ready for assembly.
The platform offers a promising solution for increasing the accessibility of custom DNA for biological research.