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Collagen toxicity in rabbits.
1Department of Biochemistry, Faculty of Science, Kuwait University, Safat.
Toxicology Letters
|April 1, 1991
Summary
Intravenous collagen infusions caused severe adverse effects, including respiratory distress and death, in animal models. Liver and lung damage were observed, indicating significant toxicity regardless of collagen source.
Area of Science:
- Biochemistry
- Toxicology
- Pathology
Background:
- Soluble collagen is used in various biomedical applications.
- Understanding collagen's in vivo effects is crucial for safety assessments.
Purpose of the Study:
- To investigate the toxic effects of different soluble collagen preparations administered intravenously.
- To determine the impact of collagen source on its toxicity profile.
Main Methods:
- Intravenous infusion of soluble collagen from various animal sources (rabbit skin, rat skin, rat tail tendon, guinea-pig skin) into lethal doses.
- Analysis of plasma enzyme levels, specifically lactate dehydrogenase (LDH).
- Histopathological examination of lungs, livers, and hearts.
Main Results:
- Collagen infusions induced respiratory distress, agitation, convulsions, and death.
- Significant increases in total, cardiac, and hepatic LDH levels were observed.
- Histopathology confirmed lung and liver damage in treated animals; heart tissues remained normal.
- The origin of the collagen did not influence the observed tissue-level toxicity.
Conclusions:
- Soluble collagen, irrespective of its source, exhibits significant intravenous toxicity.
- Collagen administration can lead to multi-organ damage, particularly affecting the lungs and liver.
- Further research is needed to elucidate the mechanisms underlying collagen-induced toxicity.