Rapid pair-wise synteny analysis of large bacterial genomes using web-based GeneOrder4.0
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Updated: Jun 15, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
Published on: December 7, 2021
Padmanabhan Mahadevan1, Donald Seto
1Department of Bioinformatics and Computational Biology, 10900 University Blvd,, MSN 5B3, George Mason University, Manassas, VA 20110, USA. dseto@gmu.edu.
Background:
The growing whole genome sequence databases necessitate the development of user-friendly software tools to mine these data. Web-based tools are particularly useful to wet-bench biologists as they enable platform-independent analysis of sequence data, without having to perform complex programming tasks and software compiling.
Findings:
GeneOrder4.0 is a web-based "on-the-fly" synteny and gene order analysis tool for comparative bacterial genomics (ca. 8 Mb). It enables the visualization of synteny by plotting protein similarity scores between two genomes and it also provides visual annotation of "hypothetical" proteins from older archived genomes based on more recent annotations.
Conclusions:
The web-based software tool GeneOrder4.0 is a user-friendly application that has been updated to allow the rapid analysis of synteny and gene order in large bacterial genomes. It is developed with the wet-bench researcher in mind.
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