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Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
Published on: September 18, 2019
Overlapping roles for Yen1 and Mus81 in cellular Holliday junction processing
1Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom.
The Journal of Biological Chemistry
|February 25, 2010
Summary
Yen1 and Mus81 proteins work together to resolve DNA structures during replication fork repair in yeast. This redundancy is crucial for repairing DNA damage, but not for double-strand breaks from radiation.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Eukaryotic Holliday junction (HJ) resolvases are critical for DNA repair.
- At least three proteins cleave model HJs in vitro, but their in vivo targets remain unclear.
Purpose of the Study:
- To develop a system for monitoring in vivo HJ resolution in budding yeast.
- To investigate the in vivo roles of Yen1, Mus81, and Slx1 in HJ resolution.
Main Methods:
- Developed a quantitative system to monitor in vivo HJ resolution in budding yeast.
- Assessed the functional redundancy between Yen1, Mus81, and Slx1.
- Investigated roles in replication fork progression and double-strand break repair.
Main Results:
- Yen1 acts redundantly with Mus81, but not Slx1, in resolving model HJs in vivo.
- This functional overlap is specific to repairing lesions impeding replication fork progression.
- No overlap was observed in repairing double-strand breaks induced by ionizing radiation.
Conclusions:
- Yen1 plays a direct role in the DNA damage response.
- Overlapping HJ resolution functions of Yen1 and Mus81 are implicated in replication fork repair.
- This study clarifies the in vivo roles of specific HJ resolvases.
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