Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hormonal regulation of membrane phenotype.

S A Carlson, T D Gelehrter

    Journal of Supramolecular Structure
    |January 1, 1977
    PubMed
    Summary

    Glucocorticoids alter transformed cell membrane properties without affecting growth. Researchers developed resistant cell lines to study steroid hormone action and protease roles in membrane regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inhibition of the virulence, antibiotic resistance, and fecal shedding of multiple antibiotic-resistant Salmonella Typhimurium in broilers fed Original XPC™.

    Poultry science·2016
    Same author

    Molecular mechanisms for anthelmintic resistance in strongyle nematode parasites of veterinary importance.

    Journal of veterinary pharmacology and therapeutics·2016
    Same author

    Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena.

    Open veterinary journal·2015
    Same author

    Non-typhoidal Salmonella encephalopathy involving lipopolysaccharide in cattle.

    Veterinary microbiology·2012
    Same author

    Experimental reproduction of bovine Salmonella encephalopathy using a norepinephrine-based stress model.

    Veterinary journal (London, England : 1997)·2007
    Same author

    Klebsiella to Salmonella gene transfer within rumen protozoa: implications for antibiotic resistance and rumen defaunation.

    Veterinary microbiology·2006

    Area of Science:

    • Cell Biology
    • Endocrinology
    • Biochemistry

    Background:

    • Transformed cells exhibit altered membrane properties.
    • Glucocorticoids can modify these membrane characteristics.
    • Plasminogen activator production is a key cellular process regulated by hormones.

    Purpose of the Study:

    • To investigate the effect of glucocorticoids on rat hepatoma (HTC) cell membrane properties.
    • To isolate and characterize cell variants resistant to dexamethasone-induced inhibition of plasminogen activator production.
    • To elucidate the mechanism of steroid hormone action and the role of proteases in membrane regulation.

    Main Methods:

    • Incubation of rat hepatoma cells (HTC) with glucocorticoids.
    • Isolation of dexamethasone-resistant cell variants using an agar-fibrin overlay technique.
    • Analysis of glucocorticoid receptor status and hormone action pathways.

    Main Results:

    • Glucocorticoids altered membrane properties of HTC cells without impacting growth rate.
    • Dexamethasone-resistant variants were successfully isolated.
    • Resistance was not due to receptor defects but a later step in hormone action specific to plasminogen activator.

    Conclusions:

    • Steroid hormone action involves complex pathways beyond receptor binding.
    • Proteases play a significant role in hormonal regulation of membrane function.
    • The isolated variants are valuable tools for studying steroid hormone mechanisms and protease functions.

    Related Experiment Videos