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Updated: Jun 15, 2026
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
Global transcriptomic analysis of murine embryonic stem cell-derived brachyury(+) (T) cells
Michael Xavier Doss1, Vilas Wagh, Herbert Schulz
1Center of Physiology and Pathophysiology, Institute of Neurophysiology, and Center of Molecular Medicine, University of Cologne (CMMC), Robert-Koch Str. 39, 50931 Cologne, GermanyMax-Delbrueck-Center for Molecular Medicine - MDC, Robert-Rössle Str. 10, 13092 Berlin, GermanyInstitute of Computer Science, University of Tartu, Liivi 2, 50409 Tartu, Estonia and Quretec Ltd, Ulikooli 6a, Tartu, EstoniaDepartment of Vertebrate Genomics, Max-Planck-Institute for Molecular Genetics, Ihnestr.73, D-14195 Berlin, Germany.
Researchers identified key gene expression changes in mesodermal cells derived from embryonic stem cells (ESCs). This study defines the transcriptomic signatures of brachyury(+) cells, revealing potential roles for Larp2 and Ankrd34b in cell development.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Transcriptomics
Background:
- Brachyury is a key transcription factor in mesoderm development.
- Embryonic stem cells (ESCs) differentiate into various cell types, including mesoderm.
- Understanding mesodermal cell identity is crucial for regenerative medicine.
Purpose of the Study:
- To identify the transcriptomic signature of brachyury-expressing mesodermal cells derived from ESCs.
- To investigate the functional roles of specific genes (Larp2, Ankrd34b) in mesodermal differentiation.
- To define the genetic and cellular identities of presumptive mesodermal cells.
Main Methods:
- Generation of brachyury(+) ESCs using fluorescent protein and antibiotic resistance markers.
- Enrichment of brachyury(+) cells via puromycin selection from embryoid bodies (EBs).
- Comprehensive transcriptomic analysis (RNA sequencing) of brachyury(+) cells, control EBs, and undifferentiated ESCs.
Main Results:
- Identification of 1573 up-regulated and 1549 down-regulated transcripts in brachyury(+) cells.
- Overrepresentation of Gene Ontology terms related to cell differentiation, morphogenesis, and motility.
- Notable up-regulation of Larp2 and Ankrd34b transcripts; Larp2 knockdown affected BMP-2, Ankrd34b knockdown affected neurogenesis and adipogenesis markers.
Conclusions:
- Transcriptomic signatures of ESC-derived brachyury(+) cells provide insights into mesodermal cell identity.
- Larp2 may regulate the mesodermal marker BMP-2.
- Ankrd34b may play roles in neurogenesis and adipogenesis regulation.
