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Identification of a catalytically essential nucleophilic residue in sheep liver cytoplasmic aldehyde dehydrogenase
T M Kitson1, J P Hill, G G Midwinter
1Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand.
The Biochemical Journal
|April 1, 1991
Abstract:
Sheep liver cytoplasmic aldehyde dehydrogenase was labelled by reaction with the substrate p-nitrophenyl di[14C]methylcarbamate. After tryptic digestion and peptide fractionation the labelled residue was identified as Cys-302. This is the first unequivocal identification of the essential enzymic nucleophile in the esterase activity of aldehyde dehydrogenase. By implication, Cys-302 is probably also the residue that is acylated by aldehyde substrates and the first residue that is modified by disulfiram.