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Urinary matrix metalloproteinase-8 and -9 activities in type 2 diabetic subjects: A marker of incipient diabetic

Nynke J van der Zijl1, Roeland Hanemaaijer, Maarten E Tushuizen

  • 1Department of Endocrinology/Diabetes Center, VU University Medical Center, Amsterdam, The Netherlands. nj.vdzijl@vumc.nl

Clinical Biochemistry
|February 27, 2010
PubMed

Insights

Urinary matrix metalloproteinases (MMPs), particularly MMP-8 and MMP-9, are elevated in patients with type 2 diabetes and albuminuria, indicating a link to kidney function decline.

Area of Science:

  • Nephrology
  • Endocrinology
  • Biochemistry

Background:

  • Diabetic nephropathy (DN) is a significant complication of type 2 diabetes mellitus (T2DM).
  • Matrix metalloproteinases (MMPs) are implicated in the pathophysiological processes underlying DN.
  • Urinary MMP activity may serve as a biomarker for renal function decline in T2DM.

Purpose of the Study:

  • To investigate the relationship between urinary MMP activity and renal function in T2DM patients.
  • To compare MMP levels in T2DM patients with and without albuminuria (UA) against healthy controls (CO).
  • To identify clinical determinants associated with elevated MMP levels in T2DM.

Main Methods:

  • Quantification of MMP-2, MMP-8, and MMP-9 activity in 24-hour urine collections.
  • Study groups included T2DM patients with albuminuria (UA, n=27), T2DM patients without albuminuria (NA, n=48), and controls (CO, n=28).
  • Analysis of risk factors for DN, including age, diabetes duration, BMI, blood pressure, glucose levels, HbA1c, and renal function.

Main Results:

  • MMP-8 and MMP-9 levels were significantly higher in UA patients compared to NA and CO groups (P<0.01).
  • Elevated MMP levels were observed in 93% of UA patients, 78% of NA patients, and 46% of CO.
  • MMP-8 and MMP-9 levels were significantly associated with age, diabetes duration, BMI, systolic blood pressure, fasting plasma glucose, HbA1c, and renal function (P<0.05).

Conclusions:

  • MMP-8 and MMP-9 are elevated in T2DM patients with albuminuria, suggesting a role in DN pathogenesis.
  • MMP-9 demonstrated the strongest correlation with clinical parameters associated with diabetic nephropathy.
  • Urinary MMP activity, particularly MMP-9, may serve as a valuable biomarker for assessing renal function and DN progression in T2DM.

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