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Updated: Jun 15, 2026
10:22
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Published on: November 12, 2015
Genomics and structure/function studies of Rhabdoviridae proteins involved in replication and transcription
R Assenberg1, O Delmas, B Morin
1Division of Structural Biology and Oxford Protein Production Facility, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK.
Antiviral Research
|March 2, 2010
Summary
The VIZIER programme advanced understanding of rhabdoviruses, identifying potential drug targets by characterizing viral replication machinery. Structural studies of key proteins offer new avenues for antiviral interventions.
Area of Science:
- Virology
- Structural Biology
- Drug Discovery
Background:
- Rhabdoviruses infect various hosts, including humans, with potential for interspecies transmission.
- The VIZIER programme focused on Vesiculovirus, Ephemerovirus, and Lyssavirus genera within the Rhabdoviridae family.
Purpose of the Study:
- To identify potential new drug targets against RNA viruses through structural characterization of their replication machinery.
- To understand the origin, evolution, and diversity of rhabdoviruses.
Main Methods:
- Comprehensive structural characterization of viral replicative machinery.
- Production of recombinant viral proteins using E. coli and insect cells (baculovirus system).
- Multi-construct, multi-virus expression screening for rapid data generation.
Main Results:
- Progress in dissecting the multi-functional L protein for structural and functional studies.
- The phosphoprotein and matrix protein proved amenable to structural biology.
- New structural information obtained on viral replication proteins.
Conclusions:
- Structural characterization of rhabdovirus proteins provides insights for novel antiviral strategies.
- The VIZIER programme's approach yielded valuable structural data for drug target identification.
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