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Updated: Jun 15, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Object wave reconstruction by phase-plate transmission electron microscopy
B Gamm1, M Dries, K Schultheiss
1Laboratorium für Elektronenmikroskopie, Karlsruher Institut für Technologie (KIT), D-76128 Karlsruhe, Germany. gamm@kit.edu
Abstract:
A method is described for the reconstruction of the amplitude and phase of the object exit wave function by phase-plate transmission electron microscopy. The proposed method can be considered as in-line holography and requires three images, taken with different phase shifts between undiffracted and diffracted electrons induced by a suitable phase-shifting device. The proposed method is applicable for arbitrary object exit wave functions and non-linear image formation. Verification of the method is performed for examples of a simulated crystalline object wave function and a wave function acquired with off-axis holography. The impact of noise on the reconstruction of the wave function is investigated.
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