Triterpenoid saponins from Gypsophila altissima L

Two new triterpenoid saponins (1, 2) were isolated from the roots of Gypsophila altissima L. (Caryophyllaceae), together with a known compound (3). The structures of new saponins were established as quillaic acid 3-O-beta-D-xylopyranosyl-(1-->3)-beta-D-glucuronopyranoside (1), and 3-O-beta-D-galactopyranosyl-(1-->2)-[beta-D-xylopyranosyl-(1-->3)]-beta-D-glucuronopyranosyl quillaic acid 28-O-(6-O-acetyl)-beta-D-glucopyranosyl-(1-->3)-[beta-D-xylopyranosyl-(1-->4)]-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-fucopyranoside (2), on the basis of various spectroscopic analyses (including heteronuclear single quantum correlation (HSQC), heteronuclear multiple bond correlation (HMBC), nuclear Overhauser effect spectroscopy (NOESY), total correlation spectroscopy (TOCSY), and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS)) and chemical degradations.