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Dynamics of bacteriophage R17 probed with a long-lifetime Ru(II) metal-ligand complex
Myung Sup Kim1, Jae Hui Kim, Beng Whwa Son
1Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Pusan National University, Yangsan 626-870, Korea.
Abstract:
The metal-ligand complex, [Ru(2,2'-bipyridine)(2)(4,4'-dicarboxy-2,2'-bipyridine)](2+) (RuBDc), was used as a spectroscopic probe for studying macromolecular dynamics. RuBDc is a very photostable probe that possesses favorable photophysical properties including long lifetime, high quantum yield, large Stokes' shift, and highly polarized emission. To further show the usefulness of this luminophore for probing macromolecular dynamics, we examined the intensity and anisotropy decays of RuBDc when conjugated to R17 bacteriophage using frequency-domain fluorometry with a blue light-emitting diode (LED) as the modulated light source. The intensity decays were best fit by a sum of two exponentials, and we obtained a longer mean lifetime at 4 degrees C (
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