Related Experiment Video
Updated: Jun 15, 2026
06:40
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
Published on: March 22, 2018
Early initiation of a replication origin tethered at the nuclear periphery
Hani Ebrahimi1, E Douglas Robertson, Angela Taddei
1Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.
Journal of Cell Science
|March 4, 2010
Summary
Artificial tethering of early replication origins to the nuclear periphery does not delay their replication timing in yeast. This suggests peripheral positioning alone does not dictate late replication.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Peripheral nuclear localization of chromosomal loci is associated with late DNA replication.
- The relationship between nuclear positioning and replication timing is not fully understood.
Purpose of the Study:
- To investigate whether artificial tethering of an early-initiating replication origin to the nuclear periphery delays its replication time in budding yeast.
- To determine if peripheral positioning can impose late replication.
Main Methods:
- Artificial tethering of the early replication origin ARS607 to the nuclear periphery using Yif1, Sir4 fragment, or Yku80 constructs.
- Dense-isotope transfer method to assess replication time.
- Single-cell microscopy to analyze nuclear positioning.
Main Results:
- ARS607 replicated early even when tethered to the nuclear periphery via Yif1 or Sir4.
- Tethering via Yku80 caused only a slight delay in ARS607 replication.
- No correlation was observed between peripheral positioning and delayed replication in individual cells.
Conclusions:
- Artificial relocation of an early replication origin to the nuclear periphery does not necessarily delay its replication.
- Replication timing is not solely determined by nuclear periphery localization.
Related Concept Videos
01:29Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
02:31Replication in Eukaryotes
Overview
02:31Replication in Eukaryotes
Overview
01:29Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...