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Updated: Jun 15, 2026

09:02
Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Published on: January 8, 2015
The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products
Li-Ling Hu1, Shan-Shan Zhang, Xiao-Xia Li
1Key Lab of Ministry of Health for Hormone and Development, Tianjin Medical University Institute of Endocrinology, Tianjin 300070, China.
Molekuliarnaia Biologiia
|March 5, 2010
Abstract
No abstract available in PubMed .

