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Related Experiment Video

Updated: Jun 15, 2026

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens

Published on: June 7, 2017

Testing virucidal activity in Germany: an update.

Jochen Steinmann¹, Manfred H Wolff

¹MikroLab GmbH, Bremen, Germany.

GMS Krankenhaushygiene Interdisziplinar

|March 5, 2010

Summary

This summary is machine-generated.

Revised guidelines enhance disinfectant testing for viral efficacy. New methods incorporate specific viruses like parvovirus and Bovine Viral Diarrhea Virus (BVDV) for limited virucidal activity, improving disinfectant evaluation.

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Determining Viral Disinfection Efficacy of Hot Water Laundering

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Determining Viral Disinfection Efficacy of Hot Water Laundering

Determining Viral Disinfection Efficacy of Hot Water Laundering

Published on: June 21, 2022

Area of Science:

Microbiology
Virology
Disinfectant Efficacy Testing

Background:

The German Society for Control of Viral Diseases (DVV) and Robert Koch-Institute (RKI) revised 1982 guidelines for quantitative suspension testing of chemical disinfectants.
The introduction of "limited virucidal activity" (enveloped viruses) expanded testing but lacked clear usage definitions.
Previous guidelines omitted BSA challenge but incorporated sustained effect and interference controls.

Purpose of the Study:

To update and refine guidelines for evaluating the virucidal activity of chemical disinfectants.
To incorporate new viral surrogates and statistical requirements for more robust efficacy testing.
To address the need for standardized, practice-oriented tests for disinfectants.

Main Methods:

Quantitative suspension tests were revised, omitting the BSA challenge and adding sustained effect and interference controls.
Tests now require at least two independent batches with biometric evaluation and 95% confidence intervals.
New test viruses include parvovirus, Bovine Viral Diarrhea Virus (BVDV) for limited virucidal activity, Feline Calicivirus (FCV) for noroviruses, and Duck Hepatitis B Virus (DHBV) for HBV.

Main Results:

The revised guideline incorporates parvovirus for chemothermal inactivation and BVDV as a surrogate for enveloped viruses.
Feline Calicivirus (FCV) is used as a surrogate for noroviruses, aligning with US practice.
Duck Hepatitis B Virus (DHBV) serves as a surrogate for Hepatitis B Virus (HBV).

Conclusions:

The revised guidelines provide a more rigorous framework for assessing disinfectant virucidal activity.
There is an ongoing need to develop practice-oriented tests and harmonize definitions at the European level.
Parallel evaluation with bacteriological testing is recommended to ensure comprehensive disinfectant efficacy assessment.