Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Context-dependent selection sculpts mutational landscapes in the normal lung.

Research square·2026
Same author

A roadmap for supporting the development of advanced therapy medicinal products in a European framework.

The Lancet regional health. Europe·2026
Same author

Correction: Consumption of traditional sardinian fermented milk promotes changes in the rat gut microbiota composition and functions.

BMC microbiology·2026
Same author

The effects of time-restricted feeding on early phases of carcinogenesis in rat liver and colon.

Frontiers in nutrition·2026
Same author

Consumption of traditional Sardinian fermented milk promotes changes in the rat gut microbiota composition and functions.

BMC microbiology·2025
Same author

Dietary Rhythms and MASLD-Related Hepatocellular Carcinoma.

Cancers·2024

Related Experiment Video

Updated: Jun 15, 2026

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
09:00

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Published on: February 9, 2015

Isolation of amniotic mesenchymal stem cells.

Fabio Marongiu1, Roberto Gramignoli, Qian Sun

  • 1University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Current Protocols in Stem Cell Biology
|March 5, 2010
PubMed
Summary

Human amniotic membrane offers a source of multipotent mesenchymal stem cells (MSCs) for regenerative medicine. This study details a simple protocol for isolating and purifying these valuable amniotic mesenchymal cells.

More Related Videos

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Isolation of Umbilical Cord-Derived Mesenchymal Stem Cells with High Yields and Low Damage
04:47

Isolation of Umbilical Cord-Derived Mesenchymal Stem Cells with High Yields and Low Damage

Published on: July 5, 2024

Related Experiment Videos

Last Updated: Jun 15, 2026

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
09:00

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Published on: February 9, 2015

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Isolation of Umbilical Cord-Derived Mesenchymal Stem Cells with High Yields and Low Damage
04:47

Isolation of Umbilical Cord-Derived Mesenchymal Stem Cells with High Yields and Low Damage

Published on: July 5, 2024

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Mesenchymal stem cells (MSCs) are multipotent cells with differentiation and immunomodulatory capabilities.
  • Human amniotic membrane is a promising source for MSCs due to its accessibility and potential therapeutic applications.

Purpose of the Study:

  • To describe a simple and detailed protocol for isolating amniotic mesenchymal cells.
  • To introduce a density separation technique for purifying amniotic mesenchymal cells from epithelial cell contamination.

Main Methods:

  • Isolation of cells from human term placental amniotic membrane.
  • Density separation technique for cell purification.

Main Results:

  • A straightforward protocol for obtaining amniotic mesenchymal cells was established.
  • A simple density separation method effectively purified amniotic mesenchymal cells.

Conclusions:

  • Human amniotic membrane is a viable source for multipotent mesenchymal stem cells.
  • The described isolation and purification methods are practical for regenerative medicine applications.