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Updated: Jun 15, 2026
Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay
Published on: December 29, 2023
Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system
1Key Biotechnology Laboratory for Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agriculture Sciences (CATAS), Hainan, P.R. China.
Abstract:
Comprehensive analysis of the interactions between 10 mature proteins of Papaya ringspot virus type P (PRSV-P) was carried out based on a yeast two-hybrid system assay (YTHS). We detected 6 interactions between different viral proteins (VPg-P1, VPg-P3, VPg-CI, VPg-CP, NIaPro-CI, and NIb-P3) and 4 self-interactions (HC-Pro, VPg, NIaPro, and CP). These interactions did not show the same directionality as corresponding interactions detected in other potyviruses and consequently, a protein interaction matrix displayed different patterns. This initial map of the protein interactions of PRSV-P allows further study of various viral proteins in order to develop anew strategy to control PRSV-P infection.
