Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural insights into cauliflower mitoribosome in translation state and in association with a late assembly factor.

Nature communications·2025
Same author

Small RNA mobility and plant virus diseases.

Journal of experimental botany·2025
Same author

CLSY docking to Pol IV requires a conserved domain critical for small RNA biogenesis and transposon silencing.

Nature communications·2024
Same author

Transposition of HOPPLA in siRNA-deficient plants suggests a limited effect of the environment on retrotransposon mobility in Brachypodium distachyon.

PLoS genetics·2024
Same author

CLSY docking to Pol IV requires a conserved domain critical for small RNA biogenesis and transposon silencing.

bioRxiv : the preprint server for biology·2024
Same author

Extensive natural Agrobacterium-induced transformation in the genus Camellia.

Planta·2023

Related Experiment Video

Updated: Jun 15, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Northern blotting techniques for small RNAs.

Todd Blevins1

  • 1Pikaard Laboratory, Biology Department, Washington University, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 6, 2010
PubMed
Summary

Northern blotting effectively detects small RNAs (smRNAs) in plant and animal cells. This method is crucial for understanding smRNA roles in gene regulation and virus infection.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • RNA silencing is a fundamental biological process in eukaryotes.
  • This process is mediated by small RNAs (smRNAs) ranging from 20-25 nucleotides.
  • Detecting and analyzing these smRNAs is essential for understanding gene regulation.

Purpose of the Study:

  • To describe optimized northern blot hybridization techniques for detecting small RNAs (smRNAs).
  • To demonstrate the specificity and sensitivity of northern blotting for smRNA analysis.
  • To illustrate the application of northern blotting in studying smRNA biogenesis, particularly during DNA virus infection.

Main Methods:

  • Detailed protocol for northern blot hybridization optimized for small RNA detection.
  • Utilization of DNA oligonucleotide probes for enhanced specificity and sensitivity.

More Related Videos

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Related Experiment Videos

Last Updated: Jun 15, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

  • Analysis of endogenous smRNAs from various model plant and animal species.

    • Main Results:

    • Northern blotting successfully detected and analyzed endogenous smRNAs in diverse plant (Arabidopsis thaliana, Nicotiana benthamiana, Oryza sativa, Zea mays, Physcomitrella patens) and animal (Drosophila melanogaster, Mus musculus) models.
    • Control blots confirmed the specificity and sensitivity of the hybridization technique.
    • The study demonstrated the utility of northern blotting in dissecting small RNA biogenesis pathways, exemplified by DNA virus infection.

    Conclusions:

    • Northern blotting is a reliable and sensitive method for detecting and characterizing small RNAs in various biological systems.
    • This technique is invaluable for investigating the biogenesis and function of small RNAs in gene silencing.
    • The described protocol aids in the study of small RNA dynamics, including responses to viral infections.