Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

7.8K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.8K
RNA Interference01:23

RNA Interference

28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of a Therapeutic Dance Program Compared With Circuit Physiotherapy in Rehabilitation of Older People With Recent Acquired Brain Injury: A Randomized Controlled Feasibility Trial With Embedded Qualitative Assessment.

Archives of rehabilitation research and clinical translation·2026
Same author

Correction: Predictive value of BMD for hip and other fractures: a meta-analysis to update FRAX.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2026
Same author

Correction: A meta-analysis of previous falls and subsequent fracture risk in cohort studies.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2026
Same author

Predictive value of BMD for hip and other fractures: a meta-analysis to update FRAX.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2026
Same author

A meta-analysis of smoking and fracture risk to update the FRAX® tool.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2026
Same author

Correlation between radiofrequency echographic multi-spectrometry (REMS) and dual-energy X-ray absorptiometry (DXA): A systematic review and meta-analysis.

Bone·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 17, 2026

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

21.1K

cDNA libraries for virus-induced gene silencing.

Andrea T Todd1, Enwu Liu, Jonathan E Page

  • 1NRC Plant Biotechnology Institute, Saskatoon, SK, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|March 6, 2010
PubMed
Summary
This summary is machine-generated.

Virus-induced gene silencing (VIGS) offers a rapid method for gene function analysis in plants. This study presents a new VIGS-ready library preparation technique to efficiently screen gene functions and identify plant phenotypes.

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K
Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato
06:34

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Published on: June 9, 2009

54.0K

Related Experiment Videos

Last Updated: Feb 17, 2026

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

21.1K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K
Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato
06:34

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Published on: June 9, 2009

54.0K

Area of Science:

  • Plant molecular biology
  • Functional genomics

Background:

  • Virus-induced gene silencing (VIGS) leverages natural plant antiviral defenses for gene knockdown.
  • VIGS is a valuable tool for high-throughput functional genomics due to its speed and ease of use.

Purpose of the Study:

  • To describe a methodology for creating VIGS-ready cDNA libraries.
  • To optimize insert design for efficient VIGS screening.
  • To enrich libraries for gene inserts relevant to specific biological processes.

Main Methods:

  • Utilizing subtractive cDNA library generation.
  • Designing VIGS-ready cDNA inserts.
  • Enriching libraries for targeted gene sequences.

Main Results:

  • A methodology for producing VIGS-ready cDNA libraries was developed.
  • The method facilitates the screening of a large number of genes.
  • Phenotypes resulting from gene knockdown/knockout can be efficiently determined.

Conclusions:

  • The described methodology enhances the utility of VIGS for functional genomics.
  • This approach allows for targeted screening of gene functions related to specific biological processes.
  • Efficient VIGS library preparation is crucial for high-throughput plant gene analysis.