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Reversible Ponceau staining as a loading control alternative to actin in Western blots

Isabel Romero-Calvo1, Borja Ocón, Patricia Martínez-Moya

  • 1Department of Biochemistry, School of Pharmacy, University of Granada, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Campus de Cartuja s/n, 18071 Granada, Spain.

Analytical Biochemistry
|March 9, 2010
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.