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Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
2,2'-bipyridyl based copper complexes down regulate expression of pro-inflammatory cytokines and suppress MAPKs in
K R Rupesh1, A Moushumi Priya, B Sundarakrishnan
1Department of Biotechnology, School of Life Sciences, Pondicherry University, Pondicherry, India.
Abstract:
2,2'-bipyridyl based copper complex I: [CuC24H22N6O10] at 10 nM and complex Ia: [Cu2C32H43N8O3](PF6)4 at 7 nM exhibited 50% inhibition of lymphocyte proliferation and less than 20% cytotoxicity in peripheral blood mononuclear cells (PBMCs). Further, pro-inflammatory cytokines such as TNF-alpha and IL-1beta and pro-inflammatory mediator such as Nitric Oxide (NO) besides inducible Nitric Oxide Synthase (iNOS) were inhibited by the copper complexes. They also down regulated the expression of cyclooxegenase-2 (COX-2), yet another mediator of inflammation. Immunoblot analysis revealed the inhibitory effect of these complexes on phosphorylated forms protein kinases (MAPKs) such as ERK1/2, JNK, and p38 in mitogen induced PBMCs.
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